California State University Monterey Bay
ESSP 241L
Syllabus
Student Folders

Aug 27
 
 
Pipetting technique / Lab Safety  Worksheet due.
Sep 3
 
 

Fish Protein Fingerprinting - Extract & denature fish protein

 
Sep 10
 
 

Fish Protein Fingerprinting - Electrophorese and stain fish proteins

 
Sep 17
 
 

Fish Protein Fingerprinting - Destain & analyze gels - Summary Powerpoint

 
Sep 24
 
 

 Chromosome 16 PV92 PCR - Isolate student genomic DNA

Write-up due.
Oct 1
 
 

Chromosome 16 PV92 PCR - PCR amplify PV92 from students

 
Oct 8
 
 

Chromosome 16 PV92 PCR - Check PCR product on agarose gels.

 
Oct 15
 
 
Chromosome 16 PV92 PCR - Bioinformatics Lab/Human Genome Lab  
Oct 22
 
 
Arabidopsis/Mimulus Mutant Screening - Astronaut Soap Experiment - Bracketing Write-up due.
Oct 29
 
 
Arabidopsis/Mimulus Mutant Screening - Astronaut Soap Experiment - Bracketing analysis  
Nov 5
 
 

Biotechnology - Transform cells with plasmid and grow - Continue Arabidopsis mutant screen.

 
Nov 12
 
 

Biotechnology - Collect positives and grow - Continue Arabidopsis mutant screen.

 
Nov 19
 
 

Biotechnology - Isolate plasmid expression product (GFP) by chromatography

 
Nov 26
 
 
Thanksgiving
 
Dec 3
 
 
Arabidopsis/Mimulus Mutant Screening - Bioinformatics Project Write-up due.
Dec 10
 
 
Arabidopsis/Mimulus Mutant Screening - Bioinformatics Project  
Dec 17
 
 
Arabidopsis/Mimulus Mutant Screening - Bioinformatics Project Write-ups due.
     
     

 

 

 


Fish Protein Electrophoresis


Genotyping


Mutant Screening


Bacterial Protein Expression System


Bioinformatics and Phylogenetic Analysis

Week 1