CSUMB Bio 241L Syllabus Fall 2009
Introduction to Cell & Molecular Biology Lab
Professor: Henrik Kibak, PhD. 831-582-4144
Course Description
CSUMB Bio 241L is the laboratory course for Bio 241. You may not enroll in 241L unless you have completed or are enrolled in Bio 241.
Techniques covered include denaturing gel electrophoresis, enzyme activity assays, photoreduction assays, the polymerase chain reaction, agarose gel electrophoresis, sequence analysis, recombinant gene expression, and protein purification.
Course Outcomes:
Students will be able to understand and lead molecular biology labs that employ basic skills necessary for today’s industry, research and teaching environments.
Students will be able to design and carry out controlled laboratory experiments in biology.
Students will be able to describe and give examples of how proteins are studied in the modern molecular biology laboratory.
Students will be able to describe and give examples of how nucleic acids (DNA) are studied in the modern molecular biology laboratory.
Students will be able to connect the core concepts of molecular and cell biology with laboratory techniques and experimental protocols.
Students will be able to describe and give examples of how enzymes and redox reactions are studied in a biological context.
Students will be able to describe and give examples of how bioinformatics is used to anyalze DNA and Protein sequences.
Students will be able to describe and give examples of how pure cultures of bacteria are maintained and how expression of genes can be manipulated.
Laboratory Activities:
1. Basic lab skills and safety
2. Fish Protein electrophoresis
3. Polymerase Chain Reaction
4. Nucleic Acid Electrophoresis
5. Eukaryotic Mutant Screening
6. Human Genome analysis
7. Bacterial Transformation
8. Bacterial Gene Expression
9. Protein purification
10. Protein Sequence Analysis
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