Microscopy Worksheet Your Name: _________________________________
Before beginning, remind yourself of the things you need to be careful of when using a compound microscope.
Today we have three goals:
1. Become familiar with our microscopes, tools we will use the entire semester.
2. Gain the ability to accurately measure size under the microscope by calibrating the ocular reticule on your compound microscope.
3. Determine the diameter of the microscope "field of view" at each magnification so that we can record roughly the sizes of the organisms and features we are viewing.
At the end of the period you should turn in this completed worksheet for a grade.
| How many millimeters is a meter? _______ | How many micrometers is a meter? _______ | |
What is the symbol for meter? ______ |
How many um in one mm? _______ | |
| What is the symbol for millimeter? ______ | How many millimeters long is a microscope slide? ______ | |
| What is the symbol for micrometer? ______ | How many mm wide is a microscope slide? ______ | |
Look at your microscope slide with the letter "e." How many millimeters across is the letter "e" ( do not use a microscope to answer this question)? ______ |
||
| Objective x Ocular = Magnification | Diameter
of Field of View in mm |
How many reticular hatch marks per millimeter? | How many microns (micrometers) between two reticular hatchmarks? | |
| Objective
#1 |
_______ x ______ = ________ | __________ |
__________ |
__________ |
| Objective
#2 |
_______ x ______ = ________ | __________ |
__________ |
__________ |
Objective #3 |
_______ x ______ = ________ | __________ |
__________ |
__________ |
See the reverse of this sheet.
Set
up your microscope to look at the letter "e" using a 4x objective
and the 10x ocular lens. Then carefully draw the letter "e" at
the size it appears. Looking at the little hatch marks etched into one
of the oculars, how many graduations cover one "e" at
each magnification? How many millimeters is each graduation at
each level
of magnification?
Your final task will be to carefully draw and indicate the size in millimeters or microns
of a protozoan from one of our classroom cultures.
 40x |
100x |
| 4x multiplied by 10x = 40 times greater than life-size | 10x multiplied by 10x = 100 times greater than life-size |
| How many millimeters across is your field of view? | How many millimeters across is your field of view? |
| 400x |
____ |
| 40x multiplied by 10x = 400 times greater than life-size | Your choice... write the magnification in the corner above. |
| How many millimeters across is your field of view? | How
many millimeters across is your field of view? |