CSUMB Bio 310L - Using Cn3D
Cn3D is a powerful tool: You already know how to download and open Cn3D from Lab #1. Here is how to see which residues might be interacting between the alpha and beta hemoglobin subunits.
- 3HHB in PDB - This is a Cn3D file, so you right-click it and store it on your desktop. Then open it with Cn3D (see first lab).
- Style --> Edit Global Style --> remove helix objects and turn on side chains
- Show/Hide -->Pick Structures --> highlight only 3HHB_A and 3HHB_B.
- Select the amino terminal valine of the top (alpha) sequence and make sure it has turned yellow.
- Show/Hide --> Select By Distance --> start with 4 Angstroms.
- Carefully scan all four sequences to see which other sidechains might interact with the valine?