CSUMB Bio 310L - "Biochemical Systems Lab" Spring 2008
Course Syllabus
Bio 310L is the laboratory course for Bio 310. This upper division lab meets from 3-6pm on Mondays right after the lecture, sometimes in a computer lab but most often in a wet lab. Topics include protein structure and function, bioinformatics, enzyme purification from animal mitochondria, specific activity of enzymes, recombinant protein expression and purification, enzyme assays, and drug discovery.Jan 28 - Studying Soluble Proteins - Myoglobin Feb 4 - Protein Similarity Searching Feb 11 - Feb 18 - Protein Structure Comparison Feb 25 - Isolating Mitochondria from Mussel Gill Tissue Mar 3 - Measuring Mitochondrial ATPase Activity Mar 10 - Measuring Mitochondrial ATPase Activity Spring Break Mar 24 - Measuring Protein Concentration Mar 31 - Cesar Chavez Holiday Apr 7 - Specific Activity and Protein Gel Electrophoresis Apr 14 - Gel Analysis Apr 21 - Recombinant Protein Purification Apr 28 - Column Chromatography of Proteins May 5 - DNA Polymerase Assay May 12 - Agarose Gel Electrophoresis of DNA