CSUMB Bio 310L - "Biochemical Systems Lab" Spring 2009
Course Syllabus
Bio 310L is the laboratory course for Bio 310. This upper division lab meets from 3-6pm on Mondays right after the lecture, sometimes in a computer lab but most often in a wet lab. Topics include protein structure and function, bioinformatics, enzyme purification from animal mitochondria, specific activity of enzymes, recombinant protein expression and purification, enzyme assays, and drug discovery.Jan 27 - Set up student websites, safety. Jan 27 - Studying Soluble Proteins - Myoglobin Feb 3 - Protein Similarity Searching Feb 10 - Protein Structure Comparison Feb 17 - Fold It! Feb 24 - Isolating Mitochondria from Mussel Gill Tissue Mar 3 - Measuring Mitochondrial ATPase Activity Mar 10 - Measuring Protein Concentration Spring Break Mar 24 - Specific Activity and Protein Gel Electrophoresis Mar 31 - Cesar Chavez Holiday Apr 7 - Gel Analysis Apr 14 - Recombinant Protein Purification Apr 21 - Column Chromatography of Proteins Apr 28 - DNA Polymerase Assay May 5 - Agarose Gel Electrophoresis of Assay Products May 12 - Units of Enzyme Activity