CSUMB Bio 310L - "Biochemical Systems Lab" Spring 2010
Course Syllabus
Bio 310L is the laboratory course for Bio 310. This upper division lab meets from 3-6pm on Mondays right after the lecture, sometimes in a computer lab but most often in a wet lab. Topics include protein structure and function, bioinformatics, enzyme purification from animal mitochondria, specific activity of enzymes, recombinant protein expression and purification, enzyme assays, and drug discovery.Jan 26 - Set up student websites.
Studying Soluble Proteins - MyoglobinFeb 2 - Protein Similarity Searching Feb 9 - Protein Structure Comparison Feb 16 - Fold It! Feb 23 - Isolating Mitochondria from Mussel Gill Tissue
Measuring Mitochondrial ATPase ActivityMar 2 - Measuring Protein Concentration Mar 9 - Specific Activity and Protein Gel Electrophoresis Mar 16 - Gel Analysis Mar 24 - Spring Break Mar 30 - Furlough Apr 6 - Recombinant Protein Purification Apr 13 - Column Chromatography of Proteins Apr 20 - DNA Polymerase Assay Apr 27 - Agarose Gel Electrophoresis of Assay Products
SDS Gel ElectrophoresisMay 4 - Units of Enzyme Activity, Gel Analysis May 11 - Lab Practical