CSUMB Bio 310L - "Biochemical Systems Lab"  Spring 2010
 
Bio 310 Logo


Henrik Kibak, Ph.D.

Course Syllabus

Student Participants

 

Bio 310L is the laboratory course for Bio 310.
This upper division lab meets from 3-6pm on Mondays right after the lecture, sometimes in a computer lab but most often in a wet lab. Topics include protein structure and function, bioinformatics, enzyme purification from animal mitochondria, specific activity of enzymes, recombinant protein expression and purification, enzyme assays, and drug discovery.
 
Jan 26 - Set up student websites.
               Studying Soluble Proteins - Myoglobin
Feb  2 -  Protein Similarity Searching
Feb 9 -   Protein Structure Comparison
Feb 16 - Fold It!
Feb 23 - Isolating Mitochondria from Mussel Gill Tissue
                Measuring Mitochondrial ATPase Activity
Mar  2 - Measuring Protein Concentration
Mar 9 - Specific Activity and Protein Gel Electrophoresis
Mar 16 - Gel Analysis
Mar 24 - Spring Break
Mar 30 - Furlough
Apr  6 - Recombinant Protein Purification
Apr 13 - Column Chromatography of Proteins
Apr 20 - DNA Polymerase Assay
Apr 27 - Agarose Gel Electrophoresis of Assay Products
              SDS Gel Electrophoresis
May  4 - Units of Enzyme Activity, Gel Analysis
May 11 - Lab Practical