CSUMB Bio 310L - Features of Soluble Proteins - Myoglobin Case Study

The objectives of this first lab are to: Learn the use of SSH for file transfers. Set up your own Bio310 webpage to index your assignments. View a tutorial on myoglobin structure. Become familiar with Cn3D, a program for viewing protein structures and alignments. Post a word document containing three images you create related to myoglobin.           For the rest of the semester you will be posting your class and lab assignments to a "310" folder you create in your campus folder. Here is how you will do it. Once you have created and checked your /campus/310/ folder, visit the tutorial on myoglobin structure. View the two animations for myoglobin and then investigate the "cross section" tool. We will now prepare to use a more sophisticated tool to analyze myoglobin. You will need to download and install Cn3D from the National Center for Biotechnology Information (NCBI). A description of Cn3D. Follow in-class instructions on using Cn3D.     Here's the assignment:   Using Cn3D and this file of coordinates for Myoglobin, prepare a figure with caption of myoglobin with hydrophobic residues colored grey. Save it to a Word file on your named "myoglobin.doc" and write your name at the top of the first page. Next prepare a figure with descriptive caption highlighting the histidine that binds the iron at the center of the heme group and the distal histidine that hydrogen binds the oxygen. Finally prepare a figure with just the heme group, circling the two carboxyl groups and indicate where they are oriented (towards the solvent or towards the interior of myoglobin). Save myoglobin.doc to the 310 folder in your campus directory.