CSUMB Bio 310L - Features of Soluble Proteins - Myoglobin Case Study

Lab #2 The objectives of this second lab are to: Become familiar with the National Center for Biotechnology Information (NCBI). Retrieve the human myoglobin protein sequence. Use the human myoglobin protein sequence to retrieve related bird, reptile, and mammal proteins. Use clustal to align myoglobin sequences from air-breathing diving vertebrates. Post a word document containing an analysis of myoglobin sequences from air breathing divers compared to non-divers.           Here's the assignment: Using the NCBI website find and retrieve the FASTA format of NP_005359 (Human myoglobin). Next, follow instructions in class to use BLAST and this sequence to search the database for bird, reptile, and mammal myoglobin sequences. Prepare an alignment using ClustalW of at least two diving representatives from each of these groups, as well as this fish sequence. The fish sequence is used as a filter. Prepare an alignment using ClustalW of at least two non-diving representatives from each of these groups, as well as this fish sequence. The fish sequence is used as a filter. Prepare a WORD document called myoglobin_divers.doc with these two alignments as captioned figures. Using the information in these two figures examine and compare the sequences of diving versus non-diving vertebrates. Look at the 3-dimensional myoglobin structures you prepared last week. Which structural features of myoglobin are conserved? How do the conserved regions from diving myoglobins compare to the non-diving conserved regions? Save myoglobin_divers.doc to the 310 folder in your campus directory. Edit your 310/index.html to include a link to myoglobin_divers.doc